Molecular Epidemiology of Blastomyces dermatitidis

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Molecular epidemiology of Blastomyces dermatitidis.

The inhalation of conidia of Blastomyces dermatitidis, a fungus found in soil, causes disease in humans and animals. We studied the genetic diversity of this pathogen by extracting DNA yeasts and analyzing them with a polymerase chain reaction (PCR)-based typing system we developed, which used restriction fragment analysis of amplicons from the regions between the rDNA repeats and allowed us to...

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The unappreciated intracellular lifestyle of Blastomyces dermatitidis.

Blastomyces dermatitidis, a dimorphic fungus and the causative agent of blastomycosis, is widely considered an extracellular pathogen, with little evidence for a facultative intracellular lifestyle. We infected mice with spores, that is, the infectious particle, via the pulmonary route and studied intracellular residence, transition to pathogenic yeast, and replication inside lung cells. Nearly...

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Severe Oculofacial Sequelae of Cutaneous Blastomyces dermatitidis.

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Microecology of Blastomyces dermatitidis: the ammonia hypothesis.

The precise microecology of Blastomyces dermatitidis is unknown, but the fungus has been associated with nitrogenous waste products and rapidly changing environmental conditions. Ammonia accumulates in certain microenvironments, is toxic to most fungi, but may not be identified in processed soil samples. Ammonia tolerance of B. dermatitidis was investigated with two strains recovered in Wiscons...

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Enhanced Toxicity in Chemically Altered Blastomyces Dermatitidis.

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ژورنال

عنوان ژورنال: Clinical Infectious Diseases

سال: 2000

ISSN: 1058-4838,1537-6591

DOI: 10.1086/313649